Thursday, 20. May 2021, 16.15 p.m. 

Online-Veranstaltung – ZOOM-Meeting _{(Please let me (kathrin.schowtka@uni-jena.de) know if you would like to attend and I will forward you the relevant access information.)}

Modelling gene regulation and signal transduction at the interface of single-cell and population data

Prof. Dr. Jana Wolf
(Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Deutschland)

Signalling pathways and gene regulation are critically involved in cellular decisions how to respond to external signals and are often perturbed in disease development. While the processes have classically been studied on the level of cell populations the focus has now shifted to single cell investigations. This allowed a very detailed view revealing not only a wide range of dynamic phenomena but also the heterogeneity between cells. I will discuss gene expression and protein synthesis at the interface of cell population and single cell data. I will introduce a method for advanced differential gene expression analysis that takes the role of the cell cycle in proliferating cell populations into account. In a second approach it will be demonstrated how time-resolved single cell data can be used to derive quantitative signalling models and how to decipher the signal integration between pathways involved in genotoxic stress response.